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One young adult puma female of 2. The puma adult and one of the young females were also injected with IU dating sites for seniors 55 and over 50 days pregnant hCG h prior to LOPU to promote in vivo maturation of oocytes, while the other sister female could not be injected and served as control. All injections were conducted using pregnwnt darting technique. Animals were deprived from food 24h and water 12h in preparation for surgery.

The Aites procedure was similar to the dating sites for seniors 55 and over 50 days pregnant previously described in small ruminants.

The maturation medium consisted of M supplemented with bLH 0. Unfortunately, we were unable to obtain semen to conduct IVF as originally planned, so we resorted into vitrifying the oocytes in order to store the oocytes until semen becomes available. The in vivo matured oocytes were vitrified shortly after collection while the immature oocytes were vitrified after completing 24h in the abovedescribed IVM conditions.

In all cases, prior to vitrifiation, the oocytes were prfgnant stripped of the cumulus cells by pipetting in handling medium containing 0. Больше информации vitrification was conducted senoirs the procedure by Vajta et al. Read the publication here: Furthermore, the procedure is compatible with collecting immature or in vivo mature quality oocytes by simply including or not hCG in the hormonal stimulation regime.

We report herein the results of LOPU conducted in 3 pumas. Follicle development was stimulated by means of IU eCG 4. Interestingly, when hCG was included in the hormonal stimulation, 41 of 42 oocytes recovered showed signs of in vivo maturation expanded cumulus.

Two females were collected twice with a month interval and yielded 55 and 22 April vs. No sequels from dating sites for seniors 55 and over 50 days pregnant procedure e. Prior to release she was equipped with a GPS collar that allowed satellite tracking of movements. Our results validate LOPU as a safe reproductive procedure for the multiplication of wild felines as part of conservation strategies, specifically pumas and jaguars which are considered vulnerable in Brazil.

Laparoscopic источник статьи pick-up is a safe procedure for the collection of oocytes for preservation efforts in Pumas Puma concolor. In order to maximize the number and quality of oocytes collected by donor, it is necessary to synchronize estrus and stimulate follicular growth using hormonal protocols that vary according to species.

In wild animals, LOPU can play an important role in conservation programs for endangered species when associated with effective IVEP and has been done in several species.

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